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Experimental Optimization of Green Hydrogen Production from Phototrophic Bacteria Rhodobacter sphaeroides

Author(s):

Swetha Garimella, Archana Vimal, Ramchander Merugu* and Awanish Kumar  

Abstract:


This study utilizes Rhodobacter sphaeroides bacteria which was isolated from sewage water for photo-production of hydrogen under various cultural conditions. We have examined different carbon and nitrogen sources for hydrogen production and further established the conditions for optimum hydrogen production by R. sphaeroides. The Response Surface Methodology (RSM) was employed to optimize the hydrogen production. We found that Benzoate and Glycine promotes higher amounts of hydrogen production under anaerobic light conditions after 96h. The production was also observed to be enhanced in the presence of growth factors B12. The first level of optimization was done using Box-Behnken Design (BBD) followed by Central Composite Design (CCD) method and maximum production of hydrogen achieved was 6.8 ml/30ml and 8.12 ml/30ml respectively. The significant model predicted was a quadratic model with R2 value 0.9459. Moreover, work presented here suggests an environment-friendly approach of harvesting H2, which could meet energy demand as clean fuel via green route.

Keywords:

Rhodobacter sphaeroides; sewage water; benzoate; optimization; enhanced hydrogen production

Affiliation:

University College of Science, Mahatma Gandhi University, Nalgonda-508254, TS, Department of Biotechnology, National Institute of Technology, Raipur-492010, CG, University College of Science, Mahatma Gandhi University, Nalgonda-508254, TS, Department of Biotechnology, National Institute of Technology, Raipur-492010, CG



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